Mandibular arch of 11 dpc mouse embryo was dissected and cultured in 37 C culture base contained 950 mL/L air and 50 mL/L carbon dioxide, using serum-free BGJb culture medium.

• 分离11dpc胎鼠的下颌弓，在含有950mL/L空气、50mL/L CO_2的37℃培养箱中用无血清BGJb培养液培养。