MTT method was adopted to examine the effect of serum on bladder transitional epithelial cells and the result showed that the optimized culture media was DMEM-F12 containing 0.05 serum.

• 应用四甲基偶氮唑盐法检测不同体积分数血清对移行上皮细胞增殖的影响；显示膀胱移行上皮细胞在含有体积分数为0.；05血清的DMEM-F12培养中生长状态最佳。