METHODS:The calvarian bone of new born rats was digested and cultured.Then the cytokine,spleen cells and bone marrow cells were added to stimulate the monocytes to form osteoclasts.
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释义
方法:采用新生大鼠颅盖骨消化法培养成骨细胞,再加入细胞因子、脾细胞、骨髓细胞,刺激单核细胞向破骨细胞的分化。
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