METHODS: The separation of D-NNA and L-NNA in plasma was achieved by chiral ligand exchange method via CEC. An aqueous eluent containing aspartame (2 mmol/L) sufficiently mixed with Cu2+ was used.

• 方法采用CEC手性配体交换模式检测血浆样品中D-硝基精氨酸(D-NNA)和L-硝基精氨酸(L-NNA)；流动相为50mmol/L醋酸缓冲液[pH5.；含2mmol/Laspar-tame；1mmol/LCu2+和5%25(v/v)甲醇];；