METHODS: The indirubin was chromatographed on an aluminium oxide column and chloroform-ethyl acetate as eluting agent,eluate was recrystalized by methanol-acetone.

• 方法:采用氧化铝柱色谱法分离靛玉红,氯仿-醋酸乙酯作为洗脱剂,洗脱液用甲醇-丙酮重结晶。