METHODS: The gene encoding human BTLA was amplified by RT-PCR from PHA activated T cells,and then the target gene fragment was inserted into retrovirus vector pEGZ-Term after being digested with EcoR I and BamH I.

• 方法:通过RT-PCR法从经PHA活化的人外周血T细胞中克隆出人BTLA编码区全长基因,经EcoR I和BamHI双酶切后插入逆转录病毒载体pEGZ-Term中构建成重组载体pEGZ-Term/BTLA。