METHODS: Human arresten gene was amplified from recombinant plasmid pGEM-Arr with polymerase chain reaction (PCR), and then cloned into prokaryotic expression vector pRSET by means of recombinant gene technology.

• 方法 :利用聚合酶链式反应 (PCR) ,由我们构建的重组质粒pGEM -Arr中扩增出arresten基因 ;