METHODS: Based on the cDNA sequence of DCN reported by GenBank, DCN segment with open reading frame was cloned into plasmid UF1 (UF1-DCN) after DNA sequencing.

• 方法:根据GenBank报道的DCN基因cDNA序列,克隆含开放读码框的DCN基因片段,测序后克隆至质粒UF1(UF1·DCN),通过磷酸钙鄄DNA沉淀法共转染293细胞,产生高滴度的含DCN基因的重组腺相关病毒(rAAV·DCN)。