METHODS :The entire coding reg ions ,the splice donor /acceptor sites,and partial reg ulator y reg ions of SEMA3B and SEMA3F g ene were screened for mutations by P CR-sequencing in 21primary NPC tumors and 2NPC cell lines (CNE2and SUNE1).

• 方法:采用PCR-直接测序方法,在21例散发性NPC组织和2例NPC细胞株(CNE2,SUNE1)中对SEMA3B和SEMA3F基因的编码区、拼接位点和部分调控区进行突变检测;