MEL cells are grown in DMEM medium,induction with 2%DMSO for three days.

• 标准条件下培养MEL细胞，经2%25DMSO诱导分化三天，收集状态良好的细胞，悬浮于无血清DMEM培养基中，调整细胞浓度为5×10~7/ml。