Light is emitted when luciferase is exposed to the appropriate luciferin substrate.Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.

• 在荧光素酶中加入正确的荧光素底物就可以放出荧光，而发出的光子可以被光敏感元件，如荧光探测器或改进后的光学显微镜探测到。