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- DETECTION OF MT LSU rRNA GENE FROM PNEUMOCYSTIS IN EXPERIMENTAL INFECTED RATS, MICE AND CLINICAL SUSPICIOUS CASES 实验感染大、小鼠和临床疑似病例肺孢子虫mt LSU rRNA基因检测
- Keywords Pneumocystis;molecular phylogeny;internal transcribed spacer (ITS);5.8S ribosomal RNA gene(5.8SrRNA gene);mitochondrial large subunit rRAN gene( mt LSU rRNA gene); 肺孢子虫;分子系统发育;内转录间隔区;5.;8S核糖体RNA基因;线粒体核糖体大亚基RNA基因;
- LSU rRNA gene 核糖体核酸基因
- The sequenced DNA fragment was 357bp long, and it shared 99% homology with the PC LSU mt rRNA gene announced by Sinclair and his colleagues. 测序结果显示所得基因片段长为357hP,与GeneBank中的D有序列比对;
- The result agreed with the former result of 12S rRNA gene studies. 同时,根据扩增情况,计算了12种鹭科鸟类的遗传距离,重建系统发生树。
- Nucleotide sequences of 472~481bp for 16S rRNA gene and 658bp for COI gene were obtained, respectively. 得到的序列总长度分别为472~481bp(16S)和658bp(COI)。
- Two pairs of specific primers: Tch old and Tch new primers were designed based on the result of 18S rRNA gene sequence. 经试验证明这两对引物能够非常有效的区分吕氏泰勒虫和尤氏泰勒虫。
- The PCR used a cattle-specific primer set targeting a 223 bp fragment from the mitochondrial 12S rRNA gene. PCR方法是用一对特异性引物来扩增牛的线粒体的12SrRNA上的一段长223bp的目的片断。
- This paper deals with the PCR amplification and sequencing of mitoch-ondrial DNA 12S rRNA gene of Charybdis japonica and Thalamita prymna. 以相应引物对日本?和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。
- DNA fragment of 12S rRNA gene was amplified from the templates extracted from the membrane or bile of snake gallbladder, and sequenced subsequently. 并分别从药材蛇胆的胆衣和胆汁中提取DNA,经PCR扩增得到约400bp的12S rRNA基因片段,并测序。
- In this study, phylogenetic relationship of 7 Soleoidei species are analysed by sequence variation of both 16S rRNA gene and COI gene. 本文对7种鳎亚目(Soleoidei)鱼类线粒体16S rRNA基因片段和COI基因片段进行了序列比较和分子系统学分析。
- Genetic distances between S. grandis and S. strictus were 0.0856 (with 16S rRNA gene) and 0.1712 (with COI gene), respectively. 数据分析结果表明16S rRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0.;0856和0 1712;两竹蛏类与小刀蛏的遗传距离分别为0
- The homology of the obtained sequence from sample two and partial mitochrondrial DNA 12S rRNA gene of Ovis ammon was as high as 98%. 2号样的序列与盘羊的线粒体DNA的12S rRNA基因序列的部分片段的同源性高达98%25。
- Approximately 400 base pairs of the mitochondrial 12S rRNA gene from 24 species of Chinese ranid frogs (Table 1) were sequenced. 测定了中国蛙科动物24种和蟾蜍科的中华大蟾蜍线粒体12S rRNA基因长约400bp片段的序列。
- Objective] By sequencing of SSU rRNA gene cloning from Xinjiang cutaneous leishmaniasis pathogen (XJCLP) to provide evidence for identification of the pathogen. [目的 ]分析新疆皮肤利什曼病病原体SSUrRNA基因序列 ,为该病原体种株鉴定分析提供实验依据。
- Analysis of 16S rRNA gene sequence indicated that the alkaliphilic strain EMB2039 exhibited maximum similarity with the modal strain DSM 6307~T of Bacillus cohnii. 16 S rDNA测序及系统发育分析结果表明EMB2039与Bacillus cohnii模式菌株DSM 6307~T亲缘关系最近。
- On the basis of homology and phylognetic analysis about 16S rRNA gene (16S rDNA), it could be speculated that the strain AB1 is a novel member of the genus Natronococcus. 基于16S rDNA序列的同源性比较及系统发育学研究表明,AB1是Natronococcus属中新成员。
- Objective To study the relationship between the activity of rRNA genes and the evolution of Ph(+) CML. 目的 研究Ph染色体阳性慢性粒细胞白血病即Ph(+ )CML患者骨髓细胞rRNA基因活性的变化规律及与CML病程发展的关系 .
- From this paper, it can be seen that the primary structure of the rRNA genes was applied. 从中可以看出,核糖体RNA基因已经广泛应用于海洋动物的分子系统学研究中,但主要利用的是核糖体RNA基因的一级结构。
- PCR SSCP silver staining technique was applied to analyze the SSU rRNA gene and kDNA of cutaneous leishmaniasis pathogen (CLP) from Karamy,Xinjiang and related leishmania species:?L-infantum L.tropica? and ?L-donovani? Xinjiang "771" strain. 应用PCR-SSCP银染技术,对皮肤利什曼病(CL)病人(P17)的病原体SSUrRNA基因及kDNA进行分析。