Keywords hepatocellular carcinoma;genetic engineering;variable gene;clone;single chain Fv;expression;purify;denature;renature;bifunctional antibody;targeting therapy;

• 肝细胞肝癌;基因工程;可变区基因;克隆;单链抗体;表达;纯化;变性;复性;双功能抗体;导向治疗;