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- 融合Kan~r基因 fused kanamicin resistance gene
- 经卡那霉素(Kan)的初步筛选,获得了29株抗Kan的再生植株。 After the preliminary selection of Kan, we get 29 anti-Kan regenerated plantlets.
- 通过离体叶片再生的Kan梯度筛选实验，将转化芽的选择选压力定为40mg/l Kan。 The survival pressure of Kan for the regeneration of transgenic shoot was confirmed as 40mg/l by the gradient antibiotic resistance experiment.
- 利用农杆菌介导法,将植物表达载体pBLGT35Svp4-st和pBLGvp4-st转化苜蓿,经卡那霉素(Kan)多重筛选,获得抗性植株。 The promoter of T35S was linearized with pBLGvp4-st. Plant expresses vector pBLGT35svp4-st was constructed by enzyming, linearizing and translating. Alfalfa contained the plant expression vector pBLGT35svp4-st and pBLGvp4- st by Agrobacterium-mediated transformation and antibiotics( Kan) many filtrate.
- 目的:比较康乐霉素C(Kan)和环孢素(Cyc)对十四酰佛波醇乙酯(TPA)和伊屋诺霉素(IM),及刀豆球蛋白A(Con A)诱导淋巴细胞增殖的作用。 AIM: To compare the suppressions of kanglemycin C (Kan) with that of cyclosporine (Cyc) on lymphocyte proliferations induced by tetradecanoylphorbol acetate (TPA) with ionomycin (IM), and concanavalin A (Con A).
- 然后依次转入附加Kan 50mg/L+Cef 500mg/L的MSB_1、MSB_2、MSB_3培养基中进行筛选，获得抗性植株。 两个品种的茎尖转化率均为7.89%±。 Subsequently, the explants were placed in turn onto MSBi, MSBz , MSBs containing Kan 50mg/L and Cef 500mg/L to be screened, and the transformants were obtained with transformation frequency amounting to 7.89%25.
- 在以前的Xcc致病比较与功能基因组学研究工作中,通过EZ::TN
Tnp转座子诱变Xcc 8004获得一个插入位点在XC3814 CDS (protein coding sequence)内的突变体。 In our previous functional genomic study, Xcc 8004 was randomly inserted with a transposon EZ::TNTnp. A pathogenicity-deficient mutant with insertional site in the CDS (protein coding sequence) XC3814 was identified. - 采用正交旋转设计方法对影响芥蓝遗传转化体系的因素进行了优化研究,结果表明:影响芥蓝KanR苗率的最主要因素是Kan浓度,而预培养时间和共培养时间是芥蓝遗传转化的主要影响因素。 By the orthogonalty design,the factors which influenced the transformation of Brassica oleracea L.var. alboglabra have been studied,the results showed that the major factor for KanR frequency is Kan concentration,and the major factor for Chinese kale transformation are pre-culture time and co-culture time.
- 在建立麝香百合叶片再生体系的基础上,研究了Mn-SOD基因转化中影响叶片转化效率的多种因素,包括Kan浓度、抑菌素浓度、浸染菌液等,从而确立了麝香百合‘white elegance’的遗传转化体系。 On the base of regeneration system from leaf explant of "white elegance", several factors that affected genetic transformation of the Easter Lily cultivars were examined, including selection of Kan concentrations, the suspension of strain EHA105, concentrations of Carb, and so on. Thus a simple and efficient genetic transformation system was developed.
- 转化烟草的同时，利用农杆菌介导的浸渍法转化拟南芥(Columbia ecotype)，得到T_0代种子，将种子干燥后，于4℃放置两周，再于加有50mg/l的Kan的1/2MS上筛选阳性植株，目前这一步她在进行当中。 Arabidopsis (Columbia ecotype) was transformed by floral dip method mediated by Agrobacterial and TO seeds have been harvested. The antibiotic screening of positive Arabidopsis plant is carrying out now.
