It was found that there were a low contamination ratio with about 4. 5% in all the axillary buds were sterilized in 0.1% HgCl2 solution for 10min, in this way the phenomenon of browning could be well avoided.

• 外植体材料用0.；1%25的HgCl_2灭菌10min后接种，可使污染率降低至4