Inoculation number and medium volume tests suggested that the optimal inoculation number was 10%, and the optimal medium volume was 50mL/150mL with Erlenmeyer flask.

• 通过接种量、装液量试验，确认摇瓶培养茯苓菌丝体适宜的接种量为10%25，装液量为50mL/150mL的三角瓶。