In vitro the proliferation and differentiation of bone marrow stem cell (BMSc) after the treatment of neurotrophic factors (NTFs) will be evaluated and the possibility of applying NTFs in the bone tissue engineering will be discussed.

• 1)在细胞水平观察神经营养因子(NTFs)对体外培养的骨髓基质干细胞(BMSc)增殖和分化的作用，为神经营养因素应用于骨组织工程种子细胞研究打下基础；