In this study, with the help of the intermidiate vector pJIT163,2-5A gene and Rnase L gene were cloned into the binary plant expression vector pBINplus to obtain the plant transformation/expression vector pBIN2-5A and pBINRL.

• 本文利用2-5A基因和RNase L基因,通过中间载体pJIT163将2-5A基因和RNase L基因克隆到双元植物表达载体pBINplus上,分别构建了2-5A基因的植物表达载体pBIN2-5A和RNase L基因的植物表达载体pBINRL。