In this study, the dicistronic expression vector (pEGFP-C1) was used to be transfected into human lung cancer cell line(ASTC-a-1)and a positive clone which stably expressed GFP in high level was obtained.

• 利用常规转染方法将带有EGFP基因的质粒载体导入人肺腺癌肿瘤细胞 (ASTC a 1) ,并得到GFP稳定表达的细胞株。