In this study, TDZ was best for inducing adventitious bud from petioles in vitro, and it was easy to regenerate adventitious bud from the younger petiole.

• 试验结果表明，细胞分裂素TDZ对梅尔诺离体叶柄再生不定芽效果显著，较嫩的叶柄容易再生出不定芽，并且不同的暗培养时间对不定芽再生有很大的影响，暗培养时间30d时效果较好。