In this manuscript lipase from Pseudomonas cepacia was immobilized in sol-gel matrix and use to its transesterification of soybean oil with methanol into biodiesel was studied.

• 采用溶胶-凝胶法对脂肪酶进行了固定化,考察了固定化酶催化大豆油转酯化的生产工艺中酶用量、醇油比、含水量、反应温度、反应时间、溶剂等参数对转酯过程的影响。