In this article a modified method of in-gel digestion of protein spots excised from Coomassie brilliant blue stained two-dimensional(2-D) electrophoresis gels is introduced.

• 介绍一种简便高效的从考马斯亮蓝染色的双向电泳凝胶切取蛋白质点,通过胶内酶切制备基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)样品的方法。