In the second experimental study, the spatio-temporal dynamics of Ras/Raf activity during 0.8J/cm~2 low fluence LPLI-induced ASTC-a-1 cells proliferation was monitored in single living cell using FRET imaging based on RaichuRas genetic reporter.

• 在第二部分的实验工作中；我们利用基于探针RaichuRas的FRET技术在活细胞内实时观测了0.；8J/cm~2激光照射诱导细胞增殖过程中Ras和Raf相互作用的动态过程。