In the optimum condition: FuGENE 6:DNA=6:1, transfection of rat MSC withGFP displayed a stable and long-term GFP expression in vitro, and untransfectedMSC gradually died in G418 selecting culture.

• 在FuGENE6:DNA二6:1的优化条件下,MSC能在体外长期稳定的表达绿色荧光蛋白,未转染上的细胞在G418的筛选下逐渐死亡。