In order to mapping the FLX, homozygous flx plants (ecotype Columbia) were crossed with wild type plants (ecotype Landsberg), and the resulting F_1 plants were allowed to self-fertilize to generate F_2 population.

• 首先将突变体flx(Columbia,Col)与不同背景野生型(Landsberg,Ler)植株杂交,从1238株F_2代中选择具有突变体晚花表型的个体339株,构建了基因定位的作图群体。