In high performance liquid chromatography, it is necessary to apply multi composition gradient elution for the separation of complex samples such as environmental and biological samples.

• 根据溶质在色谱柱内的迁移规律,建立了一种利用线性梯度实验快速获得溶质保留值方程系数,然后以串行色谱响应函数为优化指标进行多台阶梯度分离条件优化的方法。