In addition, the amplified IL-15 cDNA fragment from plasmid pBS-IL15 was digested with BamH I and cloned into EcoR I (filled in)/BamH I pBV220 vector.

• 另外，将经BamHI酶切的IL-15cDNA扩增片断与经EcoRI酶切、补平、再经BamHI酶切的pBV220载体相连，构建成重组质粒pBVB-IL15。