Immunostimulatory effects of synthetic bursin (BS) on proliferation of chicken splenic lymphocytes and gene expression of interleukin-2 (IL-2) and interleukin 6 (IL-6) were studied by MTT colorimetry, RIA and semi-quantitative RT-PCR.

• 为探讨人工合成的三肽囊素(BS)作为新型免疫增强剂的作用机制,本研究采用MTT比色法、放射免疫测定法(RIA)和相对半定量RTPCR法,分别研究了BS对体外培养的鸡脾脏淋巴细胞的增殖、对白细胞介素2(IL2)和白细胞介素6(IL6)分泌及其mRNA表达水平的影响。