Immunohistochemistry was used to detect the protein expression of nNOS in lumber spinal cord (L 4-L 5), locus coeruleus (LC) and periaqueductal gray (PAG) in morphine withdrawal rats.

• 电针结束后 30min ,取大鼠脊髓和脑 ,采用免疫组织化学方法和计算机图象分析技术 ,对脊髓腰膨大 (L4 L5)、蓝斑 (locuscoeruleus ,LC)、中脑导水管周围灰质 (periaqueductalgray ,PAG)nNOS的原位表达进行观察及半定量分析。