Immune sera from rAAV-2-Ag85A immunized Balb/c mice were tested for anti-Ag85A antibodies by the ELISA method. Cytotoxicity of the T-lymphocyte (CTL) was determined using the ~(51)Cr release assay.

• rAAV-2-Ag85A免疫Balb/c小鼠,ELISA法检测血清中抗-Ag85A抗体,~(51)Cr释放分析检测细胞毒性T淋巴细胞(CTL)活性。