IgY was isolated by using the water dilution method (WD) ,and their concentration and purity were measured respectively with Bradford method and SDS-PAGE.

• 用rHpaA免疫30周龄立克体鸡,以水稀释法联合盐析法提取HpaA-IgY,纯化浓缩后用Bradford法测定其含量,SDS-PAGE电泳检测纯度,Western blot鉴定抗原特异性。