IR is often used to investigate the reaction of thermal imine because the groups of amido acid and imide possess characteristic IR spectroscopy and IR method has the merits of simpleness, convenience and speediness.

• 由于红外光谱法具有简单、方便、快速等特点，酰胺酸、酰亚胺基团又有着特定的红外吸收峰，因而成为研究热亚胺化反应最常用的测试方法。