I know the general rule that approximately 10 column volumes of mobile phase is necessary for reequilibration, but I must demonstrate and validate that the reequilibration conditions are sufficient.

• 我知道通常的规则是需要约10个柱体积的流动相来重新平衡柱子，但是我必须要验证给别人看这些重新平衡条件是充分的。