Having found that there are Osx gene segments among the known genes induced by Cbfal in our SSH result, then we checked it in MLO-Y4 cells by RT-PCR and performed similar experiments in the two osteoblasts: MC3T3-E1 and U2OS.

• 因为，在Cbfa1诱导的已知基因中有Osx存在，所以，MLO一Y4中Cbfal诱导表达新基因的分离实验进一步以RT一PCR法证实在MLO一Y4细胞中Cbfal可以诱导osx表达。