HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction (PCR) and sequenced directly.

• 艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反应（ pcr ）和直接测序。