HEK293 cells transcripted with EDG 1, a SPP receptor, were cultured and harvested, and incubated with 32 P labeled or non labeled SPP. The SPP level was determined based on competitive binding of SPP or 32 P SPP to HEK cells.

• 通过培养和收集转染SPP受体 EDG 1的HEK2 93细胞 ；与标记及非标记SPP共孵育 ；利用它们与HEK2 93细胞的竞争性结合 ；测定细胞、血清和组织中SPP含量 .