HBV genotypes were determined by type-specific primers PCR method. PreC/BCP mutations were detected by direct sequencing of PCR products,and Clustal W 1.8 software was applied to analyzing the sequences.

• 以型特异性引物PCR法检测16对乙肝疫苗阻断失败的母婴和88例阻断成功的母亲血清样本中HBV基因型；用PCR产物直接测序法检测HBV前C/ BCP突变；采用Clustal W 1.；8软件进行序列分析。