Gene immunization experiments in dogs preimmunized with an inactivated vaccine showed that the pcDNAK-VP2C2 vector containing two copies of canine-specific CpG induced higher secondary immune response than that of the pcDNA3-VP2C1 vector.

• 对于经CPV灭活苗初次免疫的毕格犬,用pcDNAK-VP2C2(含2个拷贝CpG基序)质粒免疫产生的再次免疫应答优于pcDNA3-VP2C1。