GT1-7 cells were incubated with galanin at a cohort of concentrations for different time points and GnRH released in the supernatant was determined by RIA.

• (2 )将不同浓度的甘丙肽以不同时间与GT1- 7细胞孵育 ,用RIA法测定细胞上清液中GnRH含量。