GST-MAS-CT fusion protein was then robustly induced by ITPG and purified with glutathione-Sepharose 4B beads from the lysate of E. coli BL21, which was transformed in pGEX-MAS-CT.

• 将重组质粒转入大肠杆菌BL21内；经IPTG诱导；并用glutathione-Sepharose 4B纯化；得到纯化的融合蛋白GST-MAS-CT.