GAD67 gene had been cloned from rat brian using RT-PCR and T vector techniques. The success of cloning of Glutamic acid decarboxylase 67 gene lays a good basis for construction of a recombinant expressed.

• 采用RT -PCR和T载体技术获得了大鼠脑组织中的谷氨酸脱羧酶GAD6 7基因克隆 ；为该基因的体外表达打下基础 .