Four recombinant plasmids containing different fragments of DM104 DNA were obtained by direct gene cloning in protoplasts of B. subtilis BD224 strain, with only one fragment homologous to known bmr gene of B. subtilis.

• 基因克隆获得了4个带有DM104染色体上不同片段的重组质粒，只有1个片段与已知的枯草杆菌bmr基因同源。