Four full length cDNAs of hatching enzyme genes were cloned from gynogenetic gibel carp ( Carassius auratus gibelio) by suppression subtractive hybridization, SMART cDNA synthesis and RACE PCR.

• 用抑制性差减杂交结合SMARTcDNA合成和RACE PCR技术 ,克隆得到雌核发育银鲫 (Carassiusauratusgibelio) 4种孵化酶基因的全长cDNA。