Formation of EBs and induction of nestin positive cells: Ebs formed by hanging drop culture after 4 days, 90% nestin positive cells were positive by ITSFn screening for 6 days.

• 拟胚体的形成及向巢蛋白阳性细胞的诱导:胚胎干细胞悬浮培养4d可形成拟胚体,经ITSFn筛选6d后可见90%25巢蛋白阳性细胞。