Five degenerated primers were designed according to the P-loop, Kinase-2a, Kinase-r3a, EGF domain of NBS which universally exist in cloned R genes. PCR amplifications were performed with the designed RGA primers and cDNA as templates.

• 根据已克隆R基因普遍具有的NBS结构域的P-loop、Kinase-2a、Kinase-3a、EGF区设计5条RGA简并引物，组成5对引物组合。