Finally, the purified enzyme had a single band on an SDS polyacrylamide gel, and itsmolecular mass was estimated to be about 37KD.

• 酶蛋白经过DEAESepharose、Sephadex G-75、Octyl Sepharose三步层析分离后,经SDS-PAGE电泳,得到一条明显的条带,分子量在37KD左右。