Finally, the EBs were further induced in a medium supplemented with various concentrations of selenium sodium for 20 days. The insulin-producing cell clusters (IPCCs) were stained with dithizone (DTZ) and the results were conducted image analysis.

• 对诱导后获得的胰岛素产生细胞团（insulin-producing cell clusters，IPCCs）进行双硫腙染色，染色结果进行图像处理分析。