Expression of D1x5 mRNA disapears at the fourth passage NSCs,but can be recovered by exogenous gene transfect ion and treatment with BMP-2. In the meantime, D1x5 gene can direct in vitro expanded SVZa NSCs differentiating into neurons.

• 随着传代次数的增加,到第4代时,SVZa NSCs D1x5 mRNA表达消失,而外源性D1x5 基因转染及BMP-2可以诱导其重新表达; D1x5基因能使新生大鼠来源的SVZa NSCs向神经元方向分化。