Examination of nuclear extracts revealed that young cells had a higher increase in the amount of p-Stat3 in the nuclei than in that of senescent cells, suggesting a failure to translocate phosphorylated StatS to the nucleus in senescent cells.

• 应用Northern blotting检测了年轻细胞和衰老细胞中Stat3 mRNA的表达，结果发现衰老细胞中Stat3 mRNA的表达与年轻细胞比无明显的差别，而Western blotting示衰老细胞中Stat3蛋白的第四军医大学博士研究生论文表达明显高于年轻细胞，其活性形式磷酸化的Stat3中Stat3）的表达也不低于年轻细胞；