Euplotes octocarinatus eRF1a gene was cloned into the expression vector pRSETc. The obtained plasmid pRSETC-eRF1a was transformed into E. coli BL21(DE3),and the fusion protein His6-eRF1a was expressed.

• 将八肋游仆虫第一类肽链释放因子基因eRF1a克隆入表达载体pRSETc；并在大肠杆菌Bl21(DE3)中进行诱导表达.